基于ITS2序列的降香药材及其混伪品的分子鉴定研究

侯典云, 周红, 宋经元

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (15) : 1273-1276.

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中国药学杂志 ›› 2015, Vol. 50 ›› Issue (15) : 1273-1276. DOI: 10.11669/cpj.2015.15.003
中药质量控制与安全性检测专栏

基于ITS2序列的降香药材及其混伪品的分子鉴定研究

  • 侯典云1,2, 周红2, 宋经元2*
作者信息 +

Molecular Identification of Dalbergiae Odoriferae Lignum and Its Adulterants Using the ITS2 Sequence

  • HOU Dian-yun1,2, ZHOU Hong2,SONG Jing-yuan2*
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文章历史 +

摘要

目的 应用ITS2序列对降香药材及其混伪品进行DNA条形码鉴定。方法 以降香药材及其常见混伪品为研究对象,PCR扩增降香及其混伪品的ITS2序列并进行双向测序,利用软件MEGA5.0对ITS2序列的长度、GC含量等分析比对,基于K2P模型计算遗传距离,通过中药材DNA条形码鉴定系统和构建邻接树(NJ树)法对降香药材及其混伪品进行鉴定。结果 降香药材的ITS2序列长度为216 bp,GC含量为68.5%,种内无变异位点,降香与其混伪品的种间最小遗传距离为0.009,大于降香种内最大K2P距离。结论 中药材DNA条形码鉴定系统和构建邻接树均可以准确区分降香药材及其混伪品。

Abstract

OBJECTIVE To identify Dalbergiae Odoriferae Lignum and its adulterants using DNA barcoding. METHODS ITS2 is one of the popular DNA barcoding in the identification of traditional Chinese medicine. In this paper,the ITS2 regions of Dalbergiae Odoriferae Lignum and its adulterants were amplified and sequenced bi-directional.The length and GC content of ITS2 sequence were analyzed through MEGA5.0 software. The genetic distances were computed by kimura 2-parameter (K2P) model. Dalbergiae Odoriferae Lignum and its adulterants have been identified through the species identification system for traditional Chinese medicine and neighbor-joining (NJ) phylogenetic tree. RESULTS The sequence lengths of ITS2 of Dalbergiae Odoriferae Lignum were 216 bp, and the GC content was 68.5%. The minimum K2P interspecific genetic distances of Dalbergiae Odoriferae Lignum and its adulterants were 0.009, which was larger than that of the intraspecific genetic distances of Dalbergiae Odoriferae Lignum.The Dalbergiae Odoriferae Lignum and its adulterants can be obviously identified using the Species identification System and NJ phylogenetic trees.CONCLUSION ITS2 Regions as DNA barcode can identify Dalbergiae Odoriferae Lignum and its adulterants accurately.

关键词

降香 / ITS2 / 分子鉴定 / DNA条形码 / 混伪品

Key words

Dalbergiae Odoriferae Lignum / ITS2 / molecular identification / DNA barcode / adulterants

引用本文

导出引用
侯典云, 周红, 宋经元. 基于ITS2序列的降香药材及其混伪品的分子鉴定研究[J]. 中国药学杂志, 2015, 50(15): 1273-1276 https://doi.org/10.11669/cpj.2015.15.003
HOU Dian-yun, ZHOU Hong,SONG Jing-yuan. Molecular Identification of Dalbergiae Odoriferae Lignum and Its Adulterants Using the ITS2 Sequence[J]. Chinese Pharmaceutical Journal, 2015, 50(15): 1273-1276 https://doi.org/10.11669/cpj.2015.15.003
中图分类号: R282   

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基金

国家自然科学基金资助项目(U1404829);河南省教育厅科学技术研究重点资助项目(12B180007,14A180006);河南科技大学实验开发基金资助项目(SY1415042)

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